Journal: Cell reports

Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis

doi: 10.1016/j.celrep.2024.114388

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fluorescent Mounting Media with DAPI , OriGene , E19-18.

Techniques: Recombinant, Adhesive, Purification, Blocking Assay, Amplification, Random Hexamer Labeling, Reverse Transcription, SYBR Green Assay, Sterility, Imaging, Software

Journal: Journal of Nanobiotechnology

Article Title: Targeted theranostic photoactivation on atherosclerosis

doi: 10.1186/s12951-021-01084-z

Figure Lengend Snippet: Autophagy activation and efferocytosis enhancement in advanced atheroma over time after photoactivation. a CLSM images of the double IF of autophagy marker LC3 (green) and p62 (red) at 1 day and 1 week after laser irradiation. Blue: nucleus stained with DAPI. Scale bar = 100 μm. b Quantification of the LC3- and p62-positive areas per plaque. ** P < 0.01, *** P < 0.001. c IHC analysis of MerTK expression in the plaques of the DS-Ce6-photoactivated mice and controls. Scale bar = 100 μm. d Quantification of MerTK expression per plaque area. * P < 0.05. e Representative fluorescence micrographs of the carotid plaques double-stained with cleaved caspase-3 (red) and Mac3 (green). Scale bar = 100 μm. f Cleaved caspase-3 expression per plaque area and efferocytosis quantified as macrophage-associated (yellow arrows) versus macrophage-free apoptotic cells (white arrows) in the plaques after DS-Ce6 photoactivation. * P < 0.05. Tx treatment, Mφ macrophage, AC apoptotic cells

Article Snippet: After washing with TBST, the cryosections were mounted using a fluorescence mounting medium with DAPI, and the fluorescence was observed using a Zeiss LSM 780 Meta NLO confocal microscope (Carl Zeiss).

Techniques: Activation Assay, Marker, Irradiation, Staining, Expressing, Fluorescence

Journal: bioRxiv

Article Title: Preserving endothelial integrity in human saphenous veins during preparation for coronary bypass surgery

doi: 10.1101/2023.08.25.554690

Figure Lengend Snippet: Representative images of immunofluorescent detection of endothelial markers (A) VE-cadherin and (B) eNOS expression (n=7). EC markers are shown in pink while DAPI labeled nuclei are shown in blue. Lower magnification images of the whole vessels contain pseudo-color lines indicating staining positive areas in orange and enlarged images contain orange arrows indicating staining positivity. Pink boxes indicate the amplified image area. Graphs represent the percentage of each vessel lumen determined to be positive for endothelial markers.

Article Snippet: Samples were mounted using FadeStop TM fluorescent mounting reagent with DAPI (GenuIN Biotech #272).

Techniques: Expressing, Labeling, Staining, Amplification